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snuABE4.1
(Plasmid #246013)

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  • Purpose
    snuABE4.1 composed of PhADAR(E438Q, I536S, E637F) with 10a.a truncation fused to SpCas9 nickase [R221K, N394K, H840A] via an SV40 linker and further fused to La protein.
  • Depositing Lab
    Sangsu Bae
  • Depositing Organization
    Seoul National University
    Located in the Republic of Korea
  • Publication
    Im et al bioRxiv 2025.07.19.664718 ( How to cite )
  • Sequence Information

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 246013 Standard format: Plasmid sent in bacteria as agar stab 1 $94
Add to Cart
DNA 246013-D50 50 μg of DNA in Tris buffer $495
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DNA 246013-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pCMV
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Pediculus humanus ADAR deaminase domain (E438Q, I536S, E637F mutant)
  • Species
    Pediculus humanus
  • Mutation
    E438Q, I536S, E637F, with 10 amino acids truncation
  • GenBank ID
    EEB15288.1 XM_002427981.1
  • Promoter CMV

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer T7-forward
  • 3′ sequencing primer BGH-reverse
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.07.19.664718 for bioRxiv preprint.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    snuABE4.1 was a gift from Sangsu Bae (Addgene plasmid # 246013 ; http://n2t.net/addgene:246013 ; RRID:Addgene_246013)

  • For your References section:

    Engineered ADARs enable single-nucleotide resolution DNA A-to-G editing without bystander effects. Im HW, Jeong B, Lee Y, Oh YE, CJung C, Kim YW, Uhm H, Bae S. bioRxiv 2025.07.19.664718 10.1101/2025.07.19.664718
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