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pET-28a TULP3
(Plasmid #246176)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 246176 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pET-28a
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TULP3
  • Species
    H. sapiens (human)
  • Entrez Gene
    TULP3 (a.k.a. TUBL3, MGC45295)
  • Tag / Fusion Protein
    • HIS (N terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

BL21 growth strain recommended for bacterial expression.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-28a TULP3 was a gift from Sheng-cai Lin (Addgene plasmid # 246176 ; http://n2t.net/addgene:246176 ; RRID:Addgene_246176)

  • For your References section:

    Lithocholic acid binds TULP3 to activate sirtuins and AMPK to slow down ageing. Qu Q, Chen Y, Wang Y, Wang W, Long S, Yang HY, Wu J, Li M, Tian X, Wei X, Liu YH, Xu S, Xiong J, Yang C, Wu Z, Huang X, Xie C, Wu Y, Xu Z, Zhang C, Zhang B, Feng JW, Chen J, Feng Y, Fang H, Lin L, Xie ZK, Sun B, Tian H, Yu Y, Piao HL, Xie XS, Deng X, Zhang CS, Lin SC. Nature. 2024 Dec 18. doi: 10.1038/s41586-024-08348-2. 10.1038/s41586-024-08348-2 PubMed 39695235
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