HA SEK AL pcDNA3.1 zeo (+) - SEK1 (A-L)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||24653||Standard format: Plasmid sent in bacteria as agar stab||1||$75 *|
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Vector backbonepcDNA3.1 zeo (+)
- Backbone size w/o insert (bp) 5400
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GeneMAP2K4 (a.k.a. JNKK, JNKK1, MAPKK4, MEK4, MKK4, PRKMK4, SAPKK-1, SAPKK1, SEK1, SERK1, SKK1)
/ Fusion Protein
- HA (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
There is also A->T mutation in the following sequence LMYEER*T*VEVACY that does not alter function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:HA SEK AL pcDNA3.1 zeo (+) - SEK1 (A-L) was a gift from Jim Woodgett (Addgene plasmid # 24653 ; http://n2t.net/addgene:24653 ; RRID:Addgene_24653)
For your References section:Activation of stress-activated protein kinase by MEKK1 phosphorylation of its activator SEK1. Yan M, Dai T, Deak JC, Kyriakis JM, Zon LI, Woodgett JR, Templeton DJ. Nature. . 372(6508):798-800. 10.1038/372798a0 PubMed 7997270