MutS2A-pCambia1390
(Plasmid
#246544)
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PurposeExpression of Arabidopsis MutS2A (At5g54090) cDNA in planta
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 246544 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepCambia1390
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Backbone manufacturerAbCam
- Total vector size (bp) 11208
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Vector typePlant Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameArabidopsis MutS2A (At5g54090.1) cDNA
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SpeciesA. thaliana (mustard weed)
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Insert Size (bp)2391
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GenBank IDNM_001334190.1
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (not destroyed)
- 3′ cloning site SpeI (not destroyed)
- 5′ sequencing primer TCA ATA GCC CTT TGG TCT TCT GAG
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2025.09.02.673837 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MutS2A-pCambia1390 was a gift from Daniel Sloan (Addgene plasmid # 246544 ; http://n2t.net/addgene:246544 ; RRID:Addgene_246544) -
For your References section:
Plant MutS2 proteins function in plastid ribosome quality control. Broz AK, Kodrich K, Prasad KVSK, Kuster SA, Lee S, Forsythe ES, Sloan DB. bioRxiv 2025.09.02.673837 10.1101/2025.09.02.673837