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pcDNA3.4_hTNAP
(Plasmid #246682)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 246682 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pcDNA3.4
  • Backbone manufacturer
    Thermo Fisher Scientific
  • Backbone size w/o insert (bp) 6035
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ALPL (hTNAP amino acid 18–500)
  • Species
    H. sapiens (human)
  • Entrez Gene
    ALPL (a.k.a. AP-TNAP, APTNAP, HOPS, HPPA, HPPC, HPPI, HPPO, TNALP, TNAP, TNS-ALP, TNSALP)
  • Promoter CMV
  • Tags / Fusion Proteins
    • HA signaling peptide at N-terminal (N terminal on insert)
    • C-terminal TEV protease recognition sequence followed by a Flag tag and 8× polyhistidine tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AflII (not destroyed)
  • 3′ cloning site AgeI (not destroyed)
  • 5′ sequencing primer NA
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.4_hTNAP was a gift from Lionel Ballut (Addgene plasmid # 246682 ; http://n2t.net/addgene:246682 ; RRID:Addgene_246682)

  • For your References section:

    TNAP dephosphorylates phosphocholine and phosphoethanolamine and participates in triglyceride transport from the liver to the bloodstream. Drevet Mulard E, Imam I, Coureux PD, Briolay A, Bessueille L, Tarby A, Balayssac S, Narisawa S, Lecornu F, Gilard V, Violot S, Millan JL, Rautureau GJP, Ballut L, Magne D. Commun Biol. 2025 Oct 27;8(1):1501. doi: 10.1038/s42003-025-08901-3. 10.1038/s42003-025-08901-3 PubMed 41145834
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