pCS2 xenopus cerberus
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||24679||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4100
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesX. laevis (frog)
Insert Size (bp)1399
Entrez Genecer1 (a.k.a. cer, cer-1, dand4, tCerberus, xcer-1, xcer1)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer SP6
- 3′ sequencing primer T3 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
For injection linearize with NotI and transcribe with Sp6
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCS2 xenopus cerberus was a gift from Edward De Robertis (Addgene plasmid # 24679 ; http://n2t.net/addgene:24679 ; RRID:Addgene_24679)
For your References section:Cerberus is a head-inducing secreted factor expressed in the anterior endoderm of Spemann's organizer. Bouwmeester T, Kim S, Sasai Y, Lu B, De Robertis EM. Nature. 1996 Aug 15. 382(6592):595-601. 10.1038/382595a0 PubMed 8757128