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pCS2 xenopus cerberus
(Plasmid #24679)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 24679 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cerberus
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    1399
  • Entrez Gene
    cer1 (a.k.a. cer, cer-1, dand4, tCerberus, xcer-1, xcer1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T3
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

For injection linearize with NotI and transcribe with Sp6

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2 xenopus cerberus was a gift from Edward De Robertis (Addgene plasmid # 24679 ; http://n2t.net/addgene:24679 ; RRID:Addgene_24679)
  • For your References section:

    Cerberus is a head-inducing secreted factor expressed in the anterior endoderm of Spemann's organizer. Bouwmeester T, Kim S, Sasai Y, Lu B, De Robertis EM. Nature. 1996 Aug 15. 382(6592):595-601. 10.1038/382595a0 PubMed 8757128