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pcDNA3 xenopus twisted gastrulation
(Plasmid #24680)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 24680 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    twisted gastrulation
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    970
  • Entrez Gene
    twsg1 (a.k.a. tsg, twsg, twsg1-b, MGC69051)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Bstx1 (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

Best for in situs
For sense injection:
Cut with BamHI
Transcribe with SP6

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3 xenopus twisted gastrulation was a gift from Edward De Robertis (Addgene plasmid # 24680 ; http://n2t.net/addgene:24680 ; RRID:Addgene_24680)
  • For your References section:

    The evolutionarily conserved BMP-binding protein Twisted gastrulation promotes BMP signalling. Oelgeschlager M, Larrain J, Geissert D, De Robertis EM. Nature. 2000 Jun 15. 405(6788):757-63. 10.1038/35015500 PubMed 10866189