pCS2 xenopus cerberus short
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||24681||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4100
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesX. laevis (frog)
Insert Size (bp)1200
Entrez Genecer1 (a.k.a. cer, cer-1, dand4, tCerberus, xcer-1, xcer1)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer SP6
- 3′ sequencing primer T3 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
For injection cut with NotI.
Transcribe with SP6.
The construct fuses aa 1-25 (KHSEG25) to aa 117-270 (117RRKA)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCS2 xenopus cerberus short was a gift from Edward De Robertis (Addgene plasmid # 24681 ; http://n2t.net/addgene:24681 ; RRID:Addgene_24681)
For your References section:The head inducer Cerberus is a multifunctional antagonist of Nodal, BMP and Wnt signals. Piccolo S, Agius E, Leyns L, Bhattacharyya S, Grunz H, Bouwmeester T, De Robertis EM. Nature. 1999 Feb 25. 397(6721):707-10. 10.1038/17820 PubMed 10067895
Map uploaded by the depositor.