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pMRE104
(Plasmid #246833)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 246833 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pGRG36
  • Backbone manufacturer
    Nancy Craig
  • Backbone size w/o insert (bp) 12500
  • Total vector size (bp) 13500
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    no selectable marker
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    eCFP
  • Promoter nptII promoter

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Ledermann R, Bartsch I, Remus-Emsermann MNP et al. Stable fluorescent and enzymatic tagging of Bradyrhizobium diazoef- ficiens to analyze host-plant infection and colonization. Mol Plant Microbe In 2015;28:959–67.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMRE104 was a gift from David Drissner & Mitja Remus-Emsermann (Addgene plasmid # 246833 ; http://n2t.net/addgene:246833 ; RRID:Addgene_246833)

  • For your References section:

    MiniTn7-transposon delivery vectors for inducible or constitutive fluorescent protein expression in Enterobacteriaceae. Remus-Emsermann MN, Gisler P, Drissner D. FEMS Microbiol Lett. 2016 Aug;363(16). pii: fnw178. doi: 10.1093/femsle/fnw178. Epub 2016 Jul 20. 10.1093/femsle/fnw178 PubMed 27445318
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