pMRE107
(Plasmid
#246836)
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Purposefluorescent bacterial tagging with constitutive expressed cyan fluorescent mTurquoise2
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Depositing Labs
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 246836 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepGRG36
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Backbone manufacturerNancy Craig
- Backbone size w/o insert (bp) 12500
- Total vector size (bp) 13600
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)DH5alpha
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Growth instructionsno selectable marker
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namemTurquoise2
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SpeciesSynthetic
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Insert Size (bp)700
Resource Information
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A portion of this plasmid was derived from a plasmid made byLedermann R, Bartsch I, Remus-Emsermann MNP et al. Stable fluorescent and enzymatic tagging of Bradyrhizobium diazoef- ficiens to analyze host-plant infection and colonization. Mol Plant Microbe In 2015;28:959–67.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMRE107 was a gift from David Drissner & Mitja Remus-Emsermann (Addgene plasmid # 246836 ; http://n2t.net/addgene:246836 ; RRID:Addgene_246836) -
For your References section:
MiniTn7-transposon delivery vectors for inducible or constitutive fluorescent protein expression in Enterobacteriaceae. Remus-Emsermann MN, Gisler P, Drissner D. FEMS Microbiol Lett. 2016 Aug;363(16). pii: fnw178. doi: 10.1093/femsle/fnw178. Epub 2016 Jul 20. 10.1093/femsle/fnw178 PubMed 27445318