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PurposeExpression of mScarelt3-S2 fused with Lifeact. This construct is used for imaging actin filaments.
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Depositing Lab
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Depositing OrganizationInstitute of Biophysics, Chinese Academy of Sciences
Located in China -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 246873 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 246873-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 246873-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepEGFP-N1
- Backbone size w/o insert (bp) 3973
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemScarlet3-S2
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SpeciesSynthetic
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Insert Size (bp)687
- Promoter CMV
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Tag
/ Fusion Protein
- Lifeact (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site AgeI (not destroyed)
- 3′ cloning site NotI (destroyed during cloning)
- 5′ sequencing primer CMV-Forward
- 3′ sequencing primer SV40 polyA-Reverse
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The Lifeact sequence was fused in-frame with mScarlet3-S2 and inserted into the Age_/Not_ sites of pEGFP-N1.
DNA (Catalog # 246873-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 246873-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Lifeact-mScarlet3-S2 was a gift from Pingyong Xu (Addgene plasmid # 246873 ; http://n2t.net/addgene:246873 ; RRID:Addgene_246873) -
For your References section:
A highly photostable monomeric red fluorescent protein for dual-color 3D STED and time-lapse 3D SIM imaging. Ding Y, He W, Wang K, Xue F, Zheng K, Zhao S, Qi D, Li T, Xia P, Pan L, Chen L, Chen Z, Yuan L, Xu P. Nat Methods. 2025 Nov 26. doi: 10.1038/s41592-025-02962-2. 10.1038/s41592-025-02962-2 PubMed 41299071
