pMNRNA-T7-hMDM4-S367A
(Plasmid
#247025)
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PurposeThis plasmid is designed for in vitro transcription (IVT) using a T7 promoter, followed by expression of a human MDM4 variant carrying the S367A mutation.
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Depositing Lab
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Depositing OrganizationKyoto University, Center for iPS Cell Research and Application
Located in Japan -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 247025 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepMNRNA (synthesized)
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Vector typeBacterial Expression ; IVT
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMDM4-S367A
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Alt nameMDMX-S367A
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SpeciesH. sapiens (human)
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GenBank IDNM_002393.5
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Entrez GeneMDM4 (a.k.a. BMFS6, HDMX, MDMX, MRP1)
- Promoter T7
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer GCTTTGATCATGCCGGTAG
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMNRNA-T7-hMDM4-S367A was a gift from Masato Nakagawa (Addgene plasmid # 247025 ; http://n2t.net/addgene:247025 ; RRID:Addgene_247025) -
For your References section:
MDM4 enables efficient human iPS cell generation from PBMCs using synthetic RNAs. Nakagawa M, Nogi M, Doi H, Hayashi R, Katayama T, Ohno H, Mochizuki M, Hayashi K, Saito H. Sci Rep. 2025 Sep 8;15(1):30620. doi: 10.1038/s41598-025-16446-y. 10.1038/s41598-025-16446-y PubMed 40921827