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pET24a-VHH-tev-4xTS
(Plasmid #247150)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 247150 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pET24a
  • Backbone manufacturer
    Merck Millipore
  • Backbone size w/o insert (bp) 5500
  • Total vector size (bp) -16
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    VHH-tev-4xTS
  • Alt name
    VHH GFP
  • Species
    camelid anti-GFP nanobody
  • Promoter T7
  • Tags / Fusion Proteins
    • HA-tag (C terminal on insert)
    • His6 (C terminal on insert)
    • T7-tag (N terminal on insert)
    • BAP epitope (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SpeI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer T7-f primer
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET24a-VHH-tev-4xTS was a gift from Dominik Buser (Addgene plasmid # 247150 ; http://n2t.net/addgene:247150 ; RRID:Addgene_247150)

  • For your References section:

    Golgi enzymes are retrieved from the plasma membrane to the trans-Golgi network. Buser DP, Junne T. FEBS Lett. 2026 Aug 15. doi: 10.1002/1873-3468.70438. 10.1002/1873-3468.70438 PubMed 42603102
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