Dual_Reporter_SMN2_WT_Backbone
(Plasmid
#247173)
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Purpose(Empty Backbone) Backbone reporter plasmid for exon skipping MPRA. The construct contains Citrine exon 1, SMN2 intron 6, SMN2 intron 7, and Citrine exon 2.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 247173 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backboneDual_Reporter_SMN2_WT_Backbone
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Modifications to backboneNone
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Vector typeMammalian Expression, Synthetic Biology
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2025.10.12.681955 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Dual_Reporter_SMN2_WT_Backbone was a gift from Georg Seelig (Addgene plasmid # 247173 ; http://n2t.net/addgene:247173 ; RRID:Addgene_247173) -
For your References section:
Massively parallel assay of human splice variants reveals cis-regulatory drivers of disease-associated and cell type-specific splicing regulation. Koplik SE, Yu AM, Shelby MR, Fonseca GC, Roco CM, Zhang Y, Bogard N, Sabo AK, Rosenberg AB, Linder J, Seelig G. bioRxiv [Preprint]. 2025 Oct 13:2025.10.12.681955. doi: 10.1101/2025.10.12.681955. 10.1101/2025.10.12.681955 PubMed 41280078