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Tet-pLKO.1-puro-shCHD1_A
(Plasmid #247237)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 247237 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    Tet-pLKO-puro
  • Backbone manufacturer
    Dmitri Wiederschain (Addgene #21915)
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    shRNA targeting human CHD1
  • gRNA/shRNA sequence
    5'-CCGGGCGGTTTATCAAGAGCTATAACTCGAGTTATAGCTCTTGATAAAC-CGCTTTTTG-3'
  • Species
    H. sapiens (human)
  • Entrez Gene
    CHD1 (a.k.a. CHD-1, PILBOS)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Tet-pLKO.1-puro-shCHD1_A was a gift from Miguel Ramalho-Santos (Addgene plasmid # 247237 ; http://n2t.net/addgene:247237 ; RRID:Addgene_247237)

  • For your References section:

    CHD1 is a synthetic lethal vulnerability in MYC-driven breast cancer. Cho B, Furlan G, Lin P, Shen M, Mittal K, Mazzanti A, Penn LZ, Ramalho-Santos M. Oncogene. 2026 Apr;45(13):1198-1210. doi: 10.1038/s41388-026-03709-9. Epub 2026 Mar 7. 10.1038/s41388-026-03709-9 PubMed 41795021
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