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pMIND
(Plasmid #24730)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 24730 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pSHKLx
  • Backbone size w/o insert (bp) 6078
  • Vector type
    Bacterial Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    TetRO promoter
  • Alt name
    TetRO
  • Species
    C. glutamicum
  • Insert Size (bp)
    700

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer N/A
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMIND was a gift from Brian Robertson (Addgene plasmid # 24730 ; http://n2t.net/addgene:24730 ; RRID:Addgene_24730)
  • For your References section:

    Tetracycline-inducible gene regulation in mycobacteria. Blokpoel MC, Murphy HN, O'Toole R, Wiles S, Runn ES, Stewart GR, Young DB, Robertson BD. Nucleic Acids Res. 2005 . 33(2):e22. 10.1093/nar/gni023 PubMed 15687380