pX330-RPA194
(Plasmid #247352)

• Purpose: Expresses SpCas9 and a sgRNA targeting the human RPA194 loci for knock-in.
• Depositing Lab: Kazuhiro Maeshima
• Depositing Organization: National Institute of Genetics
  Located in Japan
• Publication: Ide et al Sci Adv. 2020 Oct 14;6(42). pii: 6/42/eabb5953. doi: 10.1126/sciadv.abb5953. Print 2020 Oct.

Backbone

• Vector backbone: pX330
• Backbone manufacturer: Feng Zhang lab (Addgene plasmid # 42230)
• Vector type: CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: RPA194
• Alt name: sgRNA targeting human RPA194
• gRNA/shRNA sequence: 5′-TGGGGTGTCGGAATTCAAAA-3′
• Species: H. sapiens (human)
• Entrez Gene: POLR1A (a.k.a. A190, AFDCIN, HLD27, RPA1, RPA190, RPA194, RPO1-4, RPO14)
• Promoter: U6

Cloning Information

• Cloning method: Golden Gate
• 5′ sequencing primer: TRC-F

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

DNA (Catalog # 247352-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 247352-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.