TH-neo donor vector
(Plasmid
#247669)
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Purposeknock-in vector to express neo from endogenous TH gene
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 247669 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonetdTomato HR vector
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Vector typeMammalian Expression ; Homology repair plasmid
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameTH-neo
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SpeciesH. sapiens (human)
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Entrez GeneTH (a.k.a. DYT14, DYT5b, TYH)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The depositor confirms that discrepancies between the depositor's sequence and Addgene's sequences have no functional consequences. Addgene NGS finds 6 amino acids (LKTKLE) between P2A and NeoR/KanR deleted, and E182D in NeoR/KanR. Addgene NGS finds a 1 nt insertion in the 5’ homology arm (NG_008128.1).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
TH-neo donor vector was a gift from Ellen Sidransky (Addgene plasmid # 247669 ; http://n2t.net/addgene:247669 ; RRID:Addgene_247669) -
For your References section:
Evaluation of Induced Pluripotent Stem Cell-Derived Dopaminergic Neurons from Siblings with Gaucher Disease Discordant for Parkinsonism. Hertz E, Rytel K, Perez G, Hao Y, Li Z, Ma C, Andersh K, Qi YA, Ryan E, Lopez G, Tayebi N, Sidransky E, Chen Y. Mov Disord. 2025 Aug;40(8):1719-1724. doi: 10.1002/mds.30273. Epub 2025 Jun 26. 10.1002/mds.30273 PubMed 40568761