pLEX307-puro-miRFP680
(Plasmid
#247686)
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PurposeConstitutively expresses miRFP680 under the EF-1a promoter with puromycin selection marker.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 247686 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepLEX307
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Backbone manufacturerDavid Root (Addgene #41392)
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Vector typeMammalian Expression, Lentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemiRFP680
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SpeciesSynthetic
- Promoter EF1a
Cloning Information
- Cloning method Gateway Cloning
Resource Information
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A portion of this plasmid was derived from a plasmid made byVladislav Verkhusha (miRFP680 is from Addgene #136557)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLEX307-puro-miRFP680 was a gift from Sho Iketani (Addgene plasmid # 247686 ; http://n2t.net/addgene:247686 ; RRID:Addgene_247686) -
For your References section:
Cross-resistance patterns in SARS-CoV-2 against 3CL protease inhibitors. Chithelen J, Lovett DH, Wang K, Torres Yanagisawa AE, Caccin FNM, Iketani S. Nat Commun. 2026 May 19;17(1):6575. doi: 10.1038/s41467-026-73444-y. 10.1038/s41467-026-73444-y PubMed 42156379