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pDONR221-SARS-CoV-2-nsp4-nsp5-C145A-nsp6
(Plasmid #247700)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 247700 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pDONR221
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SARS-CoV-2 3CL protease with C145A fused to nsp4 and nsp6
  • Species
    SARS-CoV-2
  • Mutation
    C145A

Cloning Information

  • Cloning method Gateway Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDONR221-SARS-CoV-2-nsp4-nsp5-C145A-nsp6 was a gift from Sho Iketani (Addgene plasmid # 247700 ; http://n2t.net/addgene:247700 ; RRID:Addgene_247700)

  • For your References section:

    Cross-resistance patterns in SARS-CoV-2 against 3CL protease inhibitors. Chithelen J, Lovett DH, Wang K, Torres Yanagisawa AE, Caccin FNM, Iketani S. Nat Commun. 2026 May 19;17(1):6575. doi: 10.1038/s41467-026-73444-y. 10.1038/s41467-026-73444-y PubMed 42156379
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