Srs2-C-terminus_pET28a
(Plasmid
#247774)
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PurposeThe primary use of this plasmid is as a target for CRISPR-Cas9. It can also be used to express the His-tagged C-terminus of Srs2 with Lys-to-Arg mutations.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 247774 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepET28a
- Total vector size (bp) 5549
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth TemperatureRoom Temperature
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameSrs2-C-terminus
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)231
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MutationThe C-terminus of the gene has Lys-to-Arg mutations
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GenBank IDNM_001181525.1
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Entrez GeneNEWENTRY
- Promoter T7
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7 promoter
- 3′ sequencing primer T7 terminator
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Srs2-C-terminus_pET28a was a gift from Yun-Seok Choi (Addgene plasmid # 247774 ; http://n2t.net/addgene:247774 ; RRID:Addgene_247774)
