DEST_RPS5ADIPS_OleGFP
(Plasmid
#248052)
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PurposeGateway Cloning
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 248052 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepK7WG2
- Backbone size w/o insert (bp) 8148
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Vector typePlant Expression
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Selectable markersNeomycin (select with G418) ; Oleosin1-GFP
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameArabidopsis thaliana RPS5A promoter
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Alt nameMitochondrial presequence of A. thaliana ATPase delta prime subunit; Oleosin1-GFP fusion
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SpeciesA. thaliana (mustard weed), Synthetic
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Insert Size (bp)7151
Cloning Information
- Cloning method Gateway Cloning
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2025.11.01.685978 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
DEST_RPS5ADIPS_OleGFP was a gift from Shin-ichi Arimura (Addgene plasmid # 248052 ; http://n2t.net/addgene:248052 ; RRID:Addgene_248052) -
For your References section:
Chloroplast- and Mitochondrion-Specific Random C-to-T Mutagenesis for Forward Genetics of Organelle Genomes. Kosaka N, Harada Y, Nakazato I, Okuno M, Itoh T, Tsutsumi N, Arimura SI. bioRxiv 2025.11.01.685978 10.1101/2025.11.01.685978