pLenti-Blast hsPRKAG2-b
(Plasmid
#248520)
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PurposeLentiviral vector for expression of human AMPK gamma 2 subunit short variant
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Depositing Lab
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Depositing OrganizationKarolinska Institutet
Located in Sweden -
Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 248520 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepLenti-CMV-Blast
- Backbone size w/o insert (bp) 7603
- Total vector size (bp) 8591
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Vector typeMammalian Expression
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameprotein kinase AMP-activated non-catalytic subunit gamma 2
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Alt namePRKAG2
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SpeciesH. sapiens (human)
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Insert Size (bp)990
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Entrez GenePRKAG2 (a.k.a. AAKG, AAKG2, CMH6, H91620p, WPWS)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (unknown if destroyed)
- 3′ cloning site BamHI (unknown if destroyed)
- 5′ sequencing primer CMF-F
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLenti-Blast hsPRKAG2-b was a gift from Anna Krook (Addgene plasmid # 248520 ; http://n2t.net/addgene:248520 ; RRID:Addgene_248520)