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pET29b_HEWL-VHH-cAb-Lys3-StrepII_1XFP
(Plasmid #248575)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 248575 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pET29b
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Strep-HEWL-VHH-cAb-Lys3
  • Species
    Synthetic
  • Tag / Fusion Protein
    • Strep tag II (C terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET29b_HEWL-VHH-cAb-Lys3-StrepII_1XFP was a gift from Magnus Rueping (Addgene plasmid # 248575 ; http://n2t.net/addgene:248575 ; RRID:Addgene_248575)

  • For your References section:

    Surface Plasmon Resonance (SPR) Workflow for Comparative Analysis of Nanobody Variants Binding to Lysozyme as a Model Ligand. Diaz-Galicia E, Gutu N, Peng Y, Valitova A, Renn D, Rueping M. Curr Protoc. 2026 May;6(5):e70360. doi: 10.1002/cpz1.70360. 10.1002/cpz1.70360 PubMed 42053294
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