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pCMV_HA-C_ACTL7A
(Plasmid #248683)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 248683 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pCMV-HA
  • Backbone manufacturer
    Clonetech (635690)
  • Backbone size w/o insert (bp) 5200
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Actl7a-HA
  • Alt name
    Tact2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1341
  • Entrez Gene
    Actl7a (a.k.a. Tact2)
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site KpnI (unknown if destroyed)
  • 5′ sequencing primer CAGGAAACAGCTATGACC
  • 3′ sequencing primer TAGAAGACACCGGGACCGAT
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV_HA-C_ACTL7A was a gift from Hubert Schorle (Addgene plasmid # 248683 ; http://n2t.net/addgene:248683 ; RRID:Addgene_248683)

  • For your References section:

    Actin-related protein T3 is required for acrosome biogenesis and sperm function in mice. Kovacevic A, Ordziniak E, Umer N, Arevalo L, Hinterlang LD, Ziaeipour S, Suvilla S, Merges GE, Schorle H. Development. 2026 Feb 1;153(3):dev205283. doi: 10.1242/dev.205283. Epub 2026 Feb 11. 10.1242/dev.205283 PubMed 41668650
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