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pSpyB-Gm-GFP
(Plasmid #248698)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 248698 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pSEVA631
  • Backbone manufacturer
    SEVA
  • Backbone size w/o insert (bp) 2899
  • Total vector size (bp) 3911
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamicin, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Green Fluorescent Protein
  • Alt name
    GFP
  • Alt name
    msfGFP
  • Alt name
    monomeric superfolder green fluorescent protein
  • Species
    Synthetic
  • Insert Size (bp)
    729
  • Promoter BG35

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PacI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer PS-1: AGGGCGGCGGATTTGTCC
  • 3′ sequencing primer PS-2: GCGGCAACCGAGCGTTC
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSpyB-Gm-GFP was a gift from Larry Halverson (Addgene plasmid # 248698 ; http://n2t.net/addgene:248698 ; RRID:Addgene_248698)
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