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pEM414
(Plasmid #248766)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 248766 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    p15A
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    T7Te-rrnBT1-20xTetO
  • Species
    Synthetic

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEM414 was a gift from Carolina Tropini (Addgene plasmid # 248766 ; http://n2t.net/addgene:248766 ; RRID:Addgene_248766)

  • For your References section:

    A Bacteroides synthetic biology toolkit to build an in vivo malabsorption biosensor. McCallum G, Burckhardt JC, He J, Hong A, Potvin-Trottier L, Tropini C. Cell. 2026 Feb 19;189(4):1245-1261.e21. doi: 10.1016/j.cell.2025.12.052. Epub 2026 Jan 28. 10.1016/j.cell.2025.12.052 PubMed 41610848
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