Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||24880||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4969
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)585
MutationContains aa 1-195.
Entrez GeneRCHY1 (a.k.a. ARNIP, CHIMP, PIRH2, PRO1996, RNF199, ZCHY, ZNF363)
/ Fusion Protein
- GST (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer pGEX5'
- 3′ sequencing primer pGEX3' (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
E. coli strain for expression: BL21DE3
Expression conditions: Pirh2 proteins need Zn2+ to fold properly, so 50 uM of ZnCl2 is added to the growth media and purification buffers.
Induction conditions: 0.5mM-1.0mM IPTG at RT for 3.5-4 hours.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:human pirh2-(1-195) was a gift from Cheryl Arrowsmith (Addgene plasmid # 24880 ; http://n2t.net/addgene:24880 ; RRID:Addgene_24880)
For your References section:Molecular basis of Pirh2-mediated p53 ubiquitylation. Sheng Y, Laister RC, Lemak A, Wu B, Tai E, Duan S, Lukin J, Sunnerhagen M, Srisailam S, Karra M, Benchimol S, Arrowsmith CH. Nat Struct Mol Biol. 2008 Dec . 15(12):1334-42. 10.1038/nsmb.1521 PubMed 19043414