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human pirh2-(1-195)
(Plasmid #24880)


Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 24880 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Mutation
    Contains aa 1-195.
  • Entrez Gene
    RCHY1 (a.k.a. ARNIP, CHIMP, PIRH2, PRO1996, RNF199, ZCHY, ZNF363)
  • Tag / Fusion Protein
    • GST (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer pGEX5'
  • 3′ sequencing primer pGEX3'
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

E. coli strain for expression: BL21DE3

Expression conditions: Pirh2 proteins need Zn2+ to fold properly, so 50 uM of ZnCl2 is added to the growth media and purification buffers.

Induction conditions: 0.5mM-1.0mM IPTG at RT for 3.5-4 hours.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    human pirh2-(1-195) was a gift from Cheryl Arrowsmith (Addgene plasmid # 24880 ; ; RRID:Addgene_24880)
  • For your References section:

    Molecular basis of Pirh2-mediated p53 ubiquitylation. Sheng Y, Laister RC, Lemak A, Wu B, Tai E, Duan S, Lukin J, Sunnerhagen M, Srisailam S, Karra M, Benchimol S, Arrowsmith CH. Nat Struct Mol Biol. 2008 Dec . 15(12):1334-42. 10.1038/nsmb.1521 PubMed 19043414