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EIF1a-CRE-AID / Ubc-GFP
(Plasmid #249049)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 249049 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pHAGE
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    CRE recombinase
  • Promoter EIF1alpha
  • Tag / Fusion Protein
    • Auxin inducible degron (C terminal on insert)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    GFP
  • Promoter Ubc

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site SpeI (not destroyed)
  • 3′ cloning site SpeI (not destroyed)
  • 5′ sequencing primer -
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Auxin inducible degron was PCR amplified from SINAPS vector ( a gift from Robert H. Singer, Albert Einstein college of Medicine, NY, USA; Addgene #84561) and fused to CRE C-terminal by Gibson cloning, to replace the MS2 sequence in plasmid CRE-MS2/GFP (Addgene # 219536).

Please visit https://doi.org/10.1101/2024.11.06.622258 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EIF1a-CRE-AID / Ubc-GFP was a gift from Jeffrey Gerst (Addgene plasmid # 249049 ; http://n2t.net/addgene:249049 ; RRID:Addgene_249049)

  • For your References section:

    Complementation of a human disease phenotype by intercellular mRNA transfer. Haimovich G, Dasgupta S, Govindan Ravi A, Gerst JE. Cell Rep. 2026 Sep 7;45(9):117959. doi: 10.1016/j.celrep.2026.117959. 10.1016/j.celrep.2026.117959 PubMed 42704713
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