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pKMDDV_Plasmid_2a
(Plasmid #249056)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 249056 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pJF143.J3
  • Backbone size w/o insert (bp) 4431
  • Total vector size (bp) 4979
  • Modifications to backbone
    Additional constitutive scRNA and change in origin of replication
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    scRNA
  • Species
    Synthetic
  • Insert Size (bp)
    250
  • Promoter BBa_J23119

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKMDDV_Plasmid_2a was a gift from Domitilla Del Vecchio (Addgene plasmid # 249056 ; http://n2t.net/addgene:249056 ; RRID:Addgene_249056)

  • For your References section:

    Resource competition shapes CRISPR-mediated gene activation. Aravind KM, Del Vecchio D. Cell Systems, 2026; 17 10.1016/j.cels.2025.101511
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