pSA091 PSMD14_IVS2-Cas9-BFP
(Plasmid
#249141)
-
PurposeOverexpression of SpCas9-BFP with PSMD14 IVS2 intron in 5' UTR and blasticidin resistance gene. Contains Cp36 recombination site.
-
Depositing Lab
-
Depositing OrganizationStanford University
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 249141 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 249141-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 249141-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
-
Vector backbonepPD008
-
Vector typeMammalian Expression, CRISPR ; Cp36 donor DNA
-
Selectable markersBlasticidin
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature30°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namePSMD14_IVS2-Cas9-TagBFP
-
SpeciesH. sapiens (human); S. pyogenes Cas9, synthetic
-
MutationPSMD14 IVS2 intron
-
Entrez GenePSMD14 (a.k.a. PAD1, POH1, RPN11)
- Promoter EF1a
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 249141-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 249141-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pSA091 PSMD14_IVS2-Cas9-BFP was a gift from Michael Bassik (Addgene plasmid # 249141 ; http://n2t.net/addgene:249141 ; RRID:Addgene_249141) -
For your References section:
Reduced Cas9 transgene silencing by incorporation of intron sequences. Arana S, Du PP, Vaughan-Jackson A, Enright N, Spees K, Valbuena R, Garcia CA, Nguyen T, Venida A, Seczynska M, Bintu L, Lehner PJ, Prolo LM, Bassik MC. Nat Commun. 2025 Nov 27;16(1):10656. doi: 10.1038/s41467-025-65669-0. 10.1038/s41467-025-65669-0 PubMed 41309578
