pAAV-GfaABC1D-mSLF-Myc-His
(Plasmid
#249179)
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PurposeAAV mediated overexpression of C-terminal fragment of mouse Sparcl1
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 249179 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
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SerotypeSelect serotype for details See details about
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PricingSelect serotype and quantity $ USD for preparation of µL virus + $34 USD for plasmid.
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Backbone
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Vector backbonepAAV2
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSparcl1
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SpeciesM. musculus (mouse)
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MutationC-terminal fragment
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Entrez GeneSparcl1 (a.k.a. Ecm2, Sc1, hevin, mast9)
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Tags
/ Fusion Proteins
- Myc (C terminal on insert)
- 6xHis (C terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-GfaABC1D-mSLF-Myc-His was a gift from Cagla Eroglu (Addgene plasmid # 249179 ; http://n2t.net/addgene:249179 ; RRID:Addgene_249179) -
For your References section:
Astrocyte-microglia crosstalk through Hevin and Toll-like receptor signaling controls developmental thalamocortical synapse refinement. Ramirez JJ, Hardin EJ, Sakers K, Kim J, Colon Ortiz C, Savage JT, Hanamsagar R, Block CL, Singh SK, Bilbo SD, Eroglu C. Neuron. 2026 Feb 19:S0896-6273(25)00984-5. doi: 10.1016/j.neuron.2025.12.028. 10.1016/j.neuron.2025.12.028 PubMed 41720089