pmNeptune2-Fb(GFP)
(Plasmid
#249301)
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PurposepmNeptune2-Fb(GFP) expression in mammalian cells.
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 249301 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepNIR-Fb(GFP)
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Backbone manufacturerAddgene #184675
- Backbone size w/o insert (bp) 5399
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepmNeptune2-Fb(GFP)
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SpeciesSynthetic
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Insert Size (bp)690
- Promoter CMV
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-R
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byTo make pmNeptune2-Fb(GFP), mNeptune2 was amplified from pcDNA3-mNeptune2 (Addgene #51309) and then swapped with miRFP670nano3 in the pNIR-Fb(GFP) (Addgene #184675).
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pmNeptune2-Fb(GFP) was a gift from Vladislav Verkhusha (Addgene plasmid # 249301 ; http://n2t.net/addgene:249301 ; RRID:Addgene_249301) -
For your References section:
Synthetic multicolor antigen-stabilizable nanobody platform for intersectional labeling and functional imaging. Barykina NV, Carey EM, Oliinyk OS, Mendonça-Gomes JM, de Oliveira S, Nimmerjahn A, Verkhusha VV. Nat Methods, 2026 10.1038/s41592-026-03056-3