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pTagRFP-T-Fb(ALFA)
(Plasmid #249311)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 249311 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pNIR-Fb(ALFA)
  • Backbone manufacturer
    Addgene #184678
  • Backbone size w/o insert (bp) 5414
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pTagRFP-T-Fb(ALFA)
  • Species
    Synthetic
  • Insert Size (bp)
    723
  • Promoter CMV

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    To make pTagRFP-T-Fb(ALFA), TagRFP-T was amplified from pmTagRFP-T-Mito-7 (Addgene #58023) and then swapped with mTagBFP2 in the pmTagBFP2-Fb(ALFA).

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTagRFP-T-Fb(ALFA) was a gift from Vladislav Verkhusha (Addgene plasmid # 249311 ; http://n2t.net/addgene:249311 ; RRID:Addgene_249311)

  • For your References section:

    Synthetic multicolor antigen-stabilizable nanobody platform for intersectional labeling and functional imaging. Barykina NV, Carey EM, Oliinyk OS, Mendonça-Gomes JM, de Oliveira S, Nimmerjahn A, Verkhusha VV. Nat Methods, 2026 10.1038/s41592-026-03056-3
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