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pAAV-DLX2.0-minBglobin-dTomato-Fb(LAG16)
(Plasmid #249317)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 249317 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    AAV
  • Backbone manufacturer
    Addgene #163505
  • Backbone size w/o insert (bp) 3907
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    dTomato-Fb(LAG16)
  • Species
    Synthetic
  • Insert Size (bp)
    1104
  • Promoter Beta Globin minimal promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site AscI (not destroyed)
  • 5′ sequencing primer AGCGCACGAGGGAGCTT
  • 3′ sequencing primer ACGTCGCCGTCCAGCTC
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    To make pAAV-DLX2.0-minBglobin-dTomato-Fb(LAG16), dTomato-Fb(LAG16) was amplified and inserted to the Addgene plasmid #163505 using AgeI and AscI restriction enzymes.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-DLX2.0-minBglobin-dTomato-Fb(LAG16) was a gift from Vladislav Verkhusha (Addgene plasmid # 249317 ; http://n2t.net/addgene:249317 ; RRID:Addgene_249317)

  • For your References section:

    Synthetic multicolor antigen-stabilizable nanobody platform for intersectional labeling and functional imaging. Barykina NV, Carey EM, Oliinyk OS, Mendonça-Gomes JM, de Oliveira S, Nimmerjahn A, Verkhusha VV. Nat Methods, 2026 10.1038/s41592-026-03056-3
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