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pTol2EF1a-BglobinIntron-sfGFP-Fb(BC2)
(Plasmid #249319)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 249319 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    Tol2
  • Backbone manufacturer
    Kawakami Lab
  • Backbone size w/o insert (bp) 4757
  • Vector type
    Other

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    sfGFP-Fb(BC2)
  • Species
    Synthetic
  • Insert Size (bp)
    1104
  • Promoter EF1a

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    To make pTol2EF1a-BglobinIntron-sfGFP-Fb(BC2), sfGFP-Fb(BC2) was amplified and swapped with EGFP-LC3-RFP-LC3dG in the pTol2EF1a-BglobinIntron-EGFP-LC3-RFP-LC3dG.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTol2EF1a-BglobinIntron-sfGFP-Fb(BC2) was a gift from Vladislav Verkhusha (Addgene plasmid # 249319 ; http://n2t.net/addgene:249319 ; RRID:Addgene_249319)

  • For your References section:

    Synthetic multicolor antigen-stabilizable nanobody platform for intersectional labeling and functional imaging. Barykina NV, Carey EM, Oliinyk OS, Mendonça-Gomes JM, de Oliveira S, Nimmerjahn A, Verkhusha VV. Nat Methods, 2026 10.1038/s41592-026-03056-3
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