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pNORg504
(Plasmid #249345)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 249345 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pNORg10
  • Backbone size w/o insert (bp) 6603
  • Total vector size (bp) 12501
  • Vector type
    E. coli - R. gnavus shuttle vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    sgRNA - cas9
  • Insert Size (bp)
    4382

Gene/Insert 2

  • Gene/Insert name
    homology arm for srtB4 deletion
  • Insert Size (bp)
    1600

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.08.12.669022 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNORg504 was a gift from Nozomu Obana (Addgene plasmid # 249345 ; http://n2t.net/addgene:249345 ; RRID:Addgene_249345)

  • For your References section:

    A genetic toolkit for the human gut bacterium Mediterraneibacter gnavus identifies capsular polysaccharides as a competitive colonization factor. Obana N, Nakato G, Nomura N, Fukuda S. Nat Commun. 2026 Mar 12. doi: 10.1038/s41467-026-69022-x. 10.1038/s41467-026-69022-x PubMed 41820342
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