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p_sc-eVIP-delta-Puro-EGFP-NRAS
(Plasmid #249469)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 249469 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    p_sc-eVIP-delta-puro-EGFP
  • Backbone size w/o insert (bp) 8697
  • Vector type
    Lentiviral
  • Selectable markers
    EGFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NRAS
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    573
  • Entrez Gene
    NRAS (a.k.a. ALPS4, CMNS, KRAS, N-ras, NCMS, NRAS1, NS6)
  • Promoter EF-1a

Cloning Information

  • Cloning method Gateway Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p_sc-eVIP-delta-Puro-EGFP-NRAS was a gift from Nils Blüthgen (Addgene plasmid # 249469 ; http://n2t.net/addgene:249469 ; RRID:Addgene_249469)

  • For your References section:

    Pooled single-cell screen in colorectal cancer defines transcriptional modules linked to oncogenes. Hollek V, Bohning F, Florez Vargas C, Sieber A, Morkel M, Bluthgen N. Mol Syst Biol. 2026 Jan 19. doi: 10.1038/s44320-025-00186-2. 10.1038/s44320-025-00186-2 PubMed 41555096
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