pAC51c
(Plasmid
#249664)
-
PurposeDual plasmid system for arabinose sensing in Snodgrassella alvi
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 249664 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
-
Vector backbonepAC17v5a
-
Vector typeBacterial Expression ; Arabinose biosensor
Growth in Bacteria
-
Bacterial Resistance(s)Spectinomycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameGFP
-
SpeciesSynthetic
-
Insert Size (bp)717
- Promoter pBAD25
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer atctgaatcatgcgcggatg
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pAC51c was a gift from Yolanda Schaerli (Addgene plasmid # 249664 ; http://n2t.net/addgene:249664 ; RRID:Addgene_249664)