APEX2 Fi+10
(Plasmid
#249709)
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PurposeVinculin CCMS 10 pN sensor with APEX2 readout force incensitive positive control
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 249709 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepcDNA3.1(+)
- Backbone size w/o insert (bp) 5402
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameVh-V5-AP-mutatedCC10-Vt-p2A-FLAG-dC-EX
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SpeciesSynthetic
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Insert Size (bp)4449
- Promoter CMV
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer BGH
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
APEX2 Fi+10 was a gift from Khalid Salaita (Addgene plasmid # 249709 ; http://n2t.net/addgene:249709 ; RRID:Addgene_249709)
