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pAc5.1B-EGFP-DmHPat
(Plasmid #25029)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 25029 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAc5.1B-EGFP
  • Backbone manufacturer
    Elisa Izaurralde
  • Backbone size w/o insert (bp) 6080
  • Vector type
    Drosophila expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Dh5 alpha or XL1-blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HPat
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    2907
  • GenBank ID
    NM_142335
  • Entrez Gene
    Patr-1 (a.k.a. anon-WO0118547.282, BcDNA:LD27979, CG5208, Dmel\CG5208, HPat, LD27979)
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRV (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer Ac5 forward primer site
  • 3′ sequencing primer BGH reverse priming site
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAc5.1B-EGFP-DmHPat was a gift from Elisa Izaurralde (Addgene plasmid # 25029 ; http://n2t.net/addgene:25029 ; RRID:Addgene_25029)
  • For your References section:

    HPat provides a link between deadenylation and decapping in metazoa. Haas G, Braun JE, Igreja C, Tritschler F, Nishihara T, Izaurralde E. J Cell Biol. 2010 Apr 19. 189(2):289-302. 10.1083/jcb.200910141 PubMed 20404111