Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene

pDEST53-LRRK2-WT Notes

Addgene Diagnostic Digest- EcoRI

This gel depicts the NEB 1kb ladder and a digestion of the plasmid pDEST53-LRRK2-WT with the following enzymes: EcoRI-HF.

Expected Bands
EcoRI-HF: 6142 bp, 3404 bp, 2198 bp, 1200 bp & 805bp

We observe the expected bands for this digest.

Addgene Diagnostic Digest

This gel depicts the NEB 1kb ext Ladder and a digestion of the plasmid pDEST53-LRRK2-WT with the following enzyme: EcoRV-HF

Expected bands:
EcoRV-HF: 10.5 + 3.3 kb (if wrong, may have extra band at ~4kb)

Results:
We observed the expected bands for this digest.