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HA-BRPF1
(Plasmid #250445)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 250445 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pcDNA3.1 (+)
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5394
  • Total vector size (bp) 9598
  • Modifications to backbone
    An HA tag was inserted
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    BRPF1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4202
  • GenBank ID
    NM_001003694
  • Entrez Gene
    BRPF1 (a.k.a. BR140, IDDDFP)
  • Promoter CMV
  • Tag / Fusion Protein
    • HA tag at the N-terminal

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site NotI (3-fragment ligation with BglII in the middle) (not destroyed)
  • 5′ sequencing primer T7 or CMV-F
  • 3′ sequencing primer HF-B: 5'-ACAACAGATGGCTGGCAAC-3'
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    BRPF1
  • Species
    H. sapiens (human)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HA-BRPF1 was a gift from Xiang-Jiao Yang (Addgene plasmid # 250445 ; http://n2t.net/addgene:250445 ; RRID:Addgene_250445)

  • For your References section:

    Molecular architecture of quartet MOZ/MORF histone acetyltransferase complexes. Ullah M, Pelletier N, Xiao L, Zhao SP, Wang K, Degerny C, Tahmasebi S, Cayrou C, Doyon Y, Goh SL, Champagne N, Cote J, Yang XJ. Mol Cell Biol. 2008 Nov;28(22):6828-43. doi: 10.1128/MCB.01297-08. Epub 2008 Sep 15. 10.1128/MCB.01297-08 PubMed 18794358
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