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pSC101-ΔPS-T7 Lysozyme-TEVcs-T7 RNAP-RBSBBa_B0034-geneVI
(Plasmid #250490)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 250490 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pSC101
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    Gene VI
  • Species
    M13 bacteriophage
  • Insert Size (bp)
    339

Gene/Insert 2

  • Gene/Insert name
    T7 Lysozyme-TEV cleavage site-T7 RNA polymerase
  • Species
    Synthetic
  • Insert Size (bp)
    3186
  • Promoter pKAT

Cloning Information for Gene/Insert 2

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CTAGAGTCACACAGGAAAGTACTAG
  • 3′ sequencing primer gatgcccgcgtccaggcatcaaata
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSC101-ΔPS-T7 Lysozyme-TEVcs-T7 RNAP-RBSBBa_B0034-geneVI was a gift from Mark Isalan (Addgene plasmid # 250490 ; http://n2t.net/addgene:250490 ; RRID:Addgene_250490)

  • For your References section:

    Loop engineering and activity improvement of TEV protease by a phagemid-based selection system. Yamaguchi H, Isalan M. Sci Rep (2026). doi: 10.1038/s41598-026-74102-5 10.1038/s41598-026-74102-5
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