pAAV-CMV-hTYR_T373K
(Plasmid and Viral Vector Prep for #250556)

• Purpose: Expresses inactivated form of htyr
• Depositing Lab: Miquel Vila
• Publication: pAAV-CMV-hTYR_T373K (unpublished)

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• Serotype: Select serotype for details See details about
• Pricing: Select serotype and quantity $ USD for preparation of µL virus + $34 USD for plasmid.
• How this works:
  • Place a request for a quantity of 2 (0.2 mL), 10 (1 mL), 25 (2.5 mL), or 50 (5 mL). Our all-inclusive pricing includes DNA production and QC.
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  • Receive your prep in 6–9 weeks after the MTA is approved by your organization.
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Backbone

• Vector backbone: pAAV
• Backbone manufacturer: Viral vector production unit of Vall d'Hebron Research Institute-University Autonomous of Barcelona (UPV VHIR-UAB)
• Backbone size w/o insert (bp): 5409
• Total vector size (bp): 6999
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Inactivated human Tyrosinase (T373K mutation)
• Species: H. sapiens (human)
• Insert Size (bp): 1590
• Mutation: Changed Threonine 373 to Lysine
• Entrez Gene: TYR (a.k.a. ATN, CMM8, OCA1, OCA1A, OCAIA, SHEP3)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: 5’-CGCAAATGGGCGGTAGGCGTG-3’

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

AAV1 (Catalog # 250556-AAV1)

Purpose

Ready-to-use AAV1 particles produced from pAAV-CMV-hTYR_T373K (#250556). In addition to the viral particles, you will also receive purified pAAV-CMV-hTYR_T373K plasmid DNA.

These AAV preparations are suitable purity for injection into animals.

Delivery

• Volume: 100 µL
• Titer: ≥ 1×10¹³ vg/mL
• Pricing: $1000 USD for preparation of 100 µL virus + $34 USD for plasmid.
• Storage: Store at -80℃. Thaw just before use and keep on ice.
• Shipment: Viral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Viral Production & Use

• Packaging Plasmids: encode adenoviral helper sequences and AAV rep gene, AAV1 cap gene
• Buffer: PBS + 0.001% Poloxamer 188 + 200 mM NaCl
• Serotype: AAV1
• Purification: Iodixanol gradient ultracentrifugation

Biosafety

Requestor is responsible for compliance with their institution's biosafety regulations. Lentivirus is generally considered BSL-2. AAV is generally considered BSL-1, but may require BSL-2 handling depending on the insert. Biosafety Guide

Terms and Licenses

• Academic/Nonprofit Terms:
  • Terms of Use for Viral Vectors
• Industry Terms:
  • Not Available to Industry

Viral Quality Control

Quality Control:

• Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). The specific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
• Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.

Visit our viral production page for more information.