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pGI3EM48
(Plasmid #250584)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 250584 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pGI3EM20C (Addgene 135488)
  • Vector type
    Agrobacterium binary plasmid
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    H2Apr-HygR-ADH1ter:H2Bpr-RbNLS-mYEGFP-AHD1ter
  • Species
    Spizellomyces punctatus
  • Insert Size (bp)
    2878
  • Tag / Fusion Protein
    • RbNLS (Chytrid NLS) (N terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.01.30.635136 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGI3EM48 was a gift from Lillian Fritz-Laylin (Addgene plasmid # 250584 ; http://n2t.net/addgene:250584 ; RRID:Addgene_250584)

  • For your References section:

    Cellularization in chytrid fungi uses distinct mechanisms from conventional cytokinesis and cellularization in animals and yeast. Medina EM, Elting MW, Fritz-Laylin L. Curr Biol. 2026 Jan 19;36(2):338-354.e5. doi: 10.1016/j.cub.2025.11.048. Epub 2025 Dec 15. 10.1016/j.cub.2025.11.048 PubMed 41401808
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