pTRE-ICP4-BFP-Neo
(Plasmid
#250609)
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PurposePlasmid vector expressing Tet-inducible codon-optimized HSV-1 ICP4 with a BFP reporter
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 250609 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backboneVB211009-1101fac
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Backbone manufacturerVectorBuilder
- Backbone size w/o insert (bp) 8671
- Total vector size (bp) 13258
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert 1
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Gene/Insert nameICP4
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Alt nameIE175
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Alt namevmw175
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Alt nameα4
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SpeciesHerpes simplex virus 1 (KOS strain)
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Insert Size (bp)3885
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MutationCodon-optimized for Homo sapiens
Gene/Insert 2
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Gene/Insert nameTagBFP2
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SpeciesH. sapiens (human)
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Insert Size (bp)702
Resource Information
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A portion of this plasmid was derived from a plasmid made byCustom order VB211009-1101fac from VectorBuilder.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2025.06.20.660750 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTRE-ICP4-BFP-Neo was a gift from Liam Holt (Addgene plasmid # 250609 ; http://n2t.net/addgene:250609 ; RRID:Addgene_250609)