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pTRE-ICP4-BFP-Neo
(Plasmid #250609)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 250609 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    VB211009-1101fac
  • Backbone manufacturer
    VectorBuilder
  • Backbone size w/o insert (bp) 8671
  • Total vector size (bp) 13258
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    ICP4
  • Alt name
    IE175
  • Alt name
    vmw175
  • Alt name
    α4
  • Species
    Herpes simplex virus 1 (KOS strain)
  • Insert Size (bp)
    3885
  • Mutation
    Codon-optimized for Homo sapiens

Gene/Insert 2

  • Gene/Insert name
    TagBFP2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    702

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Custom order VB211009-1101fac from VectorBuilder.

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.06.20.660750 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTRE-ICP4-BFP-Neo was a gift from Liam Holt (Addgene plasmid # 250609 ; http://n2t.net/addgene:250609 ; RRID:Addgene_250609)

  • For your References section:

    Herpes simplex virus 1 fluidizes the nucleus, enabling condensate formation. Herzog NL, Shu T, Kidiyoor GR, Keegan S, Korchi F, Chenoweth DM, Zhang H, Mohr I, Wilson AC, Holt LJ. Mol Cell. 2026 Mar 5;86(5):817-833.e7. doi: 10.1016/j.molcel.2026.02.005. 10.1016/j.molcel.2026.02.005 PubMed 41795434
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