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pLVX-TetOn-puro-ICP4-KG/NC
(Plasmid #250615)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 250615 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pLVX-TetOn-puro
  • Backbone size w/o insert (bp) 9180
  • Total vector size (bp) 13079
  • Modifications to backbone
    Insertion of two overlapping fragments of codon-optimized HSV-1 ICP4 at the MCS
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    ICP4-KG/NC
  • Alt name
    KG/NC
  • Alt name
    K463N-G464C
  • Species
    Herpes simplex virus 1 (KOS strain)
  • Insert Size (bp)
    3885
  • Mutation
    ICP4 contains two point mutations in the site-specific DNA binding domain: K463N & G464C
  • Promoter TRE3GS promoter

Cloning Information

  • Cloning method Other
  • 5′ sequencing primer cgtaccacttcctaccctcgtaaagaattcgcaggctcttaaggccaccA
  • 3′ sequencing primer tcattggctgtccagcttagctcGCAGGGGAGGTGGTCTGggagggagaggggcatcg
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Cloned codon-optimized ICP4 from Addgene #250609 (pTRE-ICP4-BFP-Neo)

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Point mutations were introduced with overlapping overhangs and NEB HiFi DNA Assembly Cloning.
Please visit https://doi.org/10.1101/2025.06.20.660750 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLVX-TetOn-puro-ICP4-KG/NC was a gift from Liam Holt (Addgene plasmid # 250615 ; http://n2t.net/addgene:250615 ; RRID:Addgene_250615)

  • For your References section:

    Herpes simplex virus 1 fluidizes the nucleus, enabling condensate formation. Herzog NL, Shu T, Kidiyoor GR, Keegan S, Korchi F, Chenoweth DM, Zhang H, Mohr I, Wilson AC, Holt LJ. Mol Cell. 2026 Mar 5;86(5):817-833.e7. doi: 10.1016/j.molcel.2026.02.005. 10.1016/j.molcel.2026.02.005 PubMed 41795434
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