GgRPE65_E148Q
(Plasmid
#250721)
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PurposeBacterial and Mammalian expression of chicken RPE65 with active site charged residue Glu148 with uncharged Gln148.
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 250721 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepCDNA3.1
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Vector typeMammalian Expression, Bacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameChicken RPE65 E148Q
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SpeciesG. gallus (chicken)
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MutationC318>T, G606>A, T963>C, C1156>T, C1161>T, G441>A, G442>C
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Entrez GeneRPE65
- Promoter T7 / CMV
Cloning Information
- Cloning method Ligation Independent Cloning
Resource Information
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A portion of this plasmid was derived from a plasmid made byPCR
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GgRPE65_E148Q was a gift from Martin Horvath (Addgene plasmid # 250721 ; http://n2t.net/addgene:250721 ; RRID:Addgene_250721)