HsRPE65
(Plasmid
#250722)
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PurposeBacterial and Mammalian expression of pure human RPE65.
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 250722 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepCDNA3.1
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Vector typeMammalian Expression, Bacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHuman RPE65
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SpeciesH. sapiens (human)
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MutationT465>C
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Entrez GeneRPE65 (a.k.a. BCO3, LCA2, RP20, mRPE65, p63, rd12, sRPE65)
- Promoter T7 / CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unspecified (unknown if destroyed)
Resource Information
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A portion of this plasmid was derived from a plasmid made byMa Group
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
HsRPE65 was a gift from Martin Horvath (Addgene plasmid # 250722 ; http://n2t.net/addgene:250722 ; RRID:Addgene_250722)